|
|
|
|
|
|
|
|
|||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | |||||
Journal of Cell Science, Vol 101, Issue 4 907-913, Copyright © 1992 by Company of Biologists
JOURNAL ARTICLES |
GJ Cannon and JA Swanson
Department of Anatomy and Cellular Biology, Harvard Medical School, MA 02115.
Murine bone marrow-derived macrophages, which measure 13.8 +/- 2.3 microns diameter in suspension, can ingest IgG-opsonized latex beads greater than 20 microns diameter. A precise assay has allowed the determination of the phagocytic capacity, and of physiological parameters that limit that capacity. Ingestion of beads larger than 15 microns diameter required IgG-opsonization, and took 30 minutes to reach completion. Despite the dependence on Fc-receptors for phagocytosis of larger beads, cells reached their limit before all cell surface Fc-receptors were occupied. The maximal membrane surface area after frustrated phagocytosis of opsonized coverslips was similar to the membrane surface area required to engulf particles at the limiting diameter, indicating that the capacity was independent of particle shape. Vacuolation of the lysosomal compartment with sucrose, which expanded endocytic compartments, lowered the phagocytic capacity. This decrease was reversed when sucrose vacuoles were collapsed by incubation of cells with invertase. These experiments indicate that the phagocytic capacity is limited by the amount of available membrane, rather than by the availability of Fc-receptors. The capacity was also reduced by depolymerization of cytoplasmic microtubules with nocodazole. Nocodazole did not affect the area of maximal cell spreading during frustrated phagocytosis, but did alter the shape of the spread cells. Thus, microtubules may coordinate cytoplasm for engulfment of the largest particles.
This article has been cited by other articles:
|
|
 |
|
  A. G. Dupuy and E. Caron Integrin-dependent phagocytosis - spreading from microadhesion to new concepts J. Cell Sci., June 1, 2008; 121(11): 1773 - 1783. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. G. Binker, D. Y. Zhao, S. J. Y. Pang, and R. E. Harrison Cytoplasmic Linker Protein-170 Enhances Spreading and Phagocytosis in Activated Macrophages by Stabilizing Microtubules J. Immunol., September 15, 2007; 179(6): 3780 - 3791. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. W. Eng, A. Bettio, J. Ibrahim, and R. E. Harrison MTOC Reorientation Occurs during Fc{gamma}R-mediated Phagocytosis in Macrophages Mol. Biol. Cell, July 1, 2007; 18(7): 2389 - 2399. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. A. Champion and S. Mitragotri From the Cover: Role of target geometry in phagocytosis PNAS, March 28, 2006; 103(13): 4930 - 4934. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Becker, A. Volchuk, and J. E. Rothman Differential use of endoplasmic reticulum membrane for phagocytosis in J774 macrophages PNAS, March 15, 2005; 102(11): 4022 - 4026. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Dzakpasu and D. Axelrod Dynamic Light Scattering Microscopy. A Novel Optical Technique to Image Submicroscopic Motions. II: Experimental Applications Biophys. J., August 1, 2004; 87(2): 1288 - 1297. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Sousa, D. Cabanes, A. El-Amraoui, C. Petit, M. Lecuit, and P. Cossart Unconventional myosin VIIa and vezatin, two proteins crucial for Listeria entry into epithelial cells J. Cell Sci., April 15, 2004; 117(10): 2121 - 2130. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. A. Beningo and Y.-l. Wang Fc-receptor-mediated phagocytosis is regulated by mechanical properties of the target J. Cell Sci., February 15, 2002; 115(4): 849 - 856. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Mancuso and M. Peters-Golden Modulation of Alveolar Macrophage Phagocytosis by Leukotrienes Is Fc Receptor-Mediated and Protein Kinase C-Dependent Am. J. Respir. Cell Mol. Biol., December 1, 2000; 23(6): 727 - 733. [Abstract] [Full Text]
|
|
|
|
 |
|
 E. S. Gold, D. M. Underhill, N. S. Morrissette, J. Guo, M. A. McNiven, and A. Aderem Dynamin 2 Is Required for Phagocytosis in Macrophages J. Exp. Med., December 20, 1999; 190(12): 1849 - 1856. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P Herbomel, B Thisse, and C Thisse Ontogeny and behaviour of early macrophages in the zebrafish embryo Development, January 9, 1999; 126(17): 3735 - 3745. [Abstract] [PDF]
|
|
|
|
|
|
D. G. Perry, G. L. Daugherty, and W. J. Martin II Clathrin-Coated Pit-Associated Proteins Are Required for Alveolar Macrophage Phagocytosis J. Immunol., January 1, 1999; 162(1): 380 - 386. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. Peracino, J. Borleis, T. Jin, M. Westphal, J.-M. Schwartz, L. Wu, E. Bracco, G. Gerisch, P. Devreotes, and S. Bozzaro G Protein beta Subunit-null Mutants Are Impaired in Phagocytosis and Chemotaxis Due to Inappropriate Regulation of the Actin Cytoskeleton J. Cell Biol., June 29, 1998; 141(7): 1529 - 1537. [Abstract] [Full Text] [PDF]
|
|
