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Journal of Cell Science, Vol 101, Issue 4 745-750, Copyright © 1992 by Company of Biologists

JOURNAL ARTICLES

Histamine decreases the permeability of an endothelial cell monolayer by stimulating cyclic AMP production through the H2-receptor

T Takeda, Y Yamashita, S Shimazaki and Y Mitsui
Division of Cell Science and Technology, Fermentation Research Institute, Agency of Industrial Science and Technology, Ibaraki, Japan.

To determine if histamine acts directly on the vascular endothelium, the effect of histamine on the permeability of cultured human endothelial cell monolayers and the role of second messengers were examined. The addition of 10(-6) to 10(-4) M histamine to the culture medium decreased the endothelial cell monolayer permeability and increased both cyclic AMP and free-calcium levels. The decrease in permeability and the increase in cyclic AMP mediated by histamine were prevented by an H2-blocker (famotidine) while the increase in free-calcium was inhibited by an H1-blocker (diphenhydramine). These results suggest that histamine decreases the permeability of endothelial cell monolayers through the H2-receptor, and cyclic AMP plays a more important role than calcium ion as a second messenger.




© The Company of Biologists Ltd 1992