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Journal of Cell Science, Vol 101, Issue 3 701-707, Copyright © 1992 by Company of Biologists
JOURNAL ARTICLES |
M Martins-Green, A Aotaki-Keen, LM Hjelmeland and MJ Bissell
Division Cell and Molecular Biology, Lawrence Berkeley Laboratory, CA 94720.
The avian gene 9E3/CEF4 belongs to a group of genes whose products are highly conserved and are homologous to inflammatory mediators. These genes, sometimes referred to as the gro family, are also expressed upon wounding or serum-stimulation of quiescent cells, suggesting that they may be important in aspects of growth and/or wound healing. We have used an antibody to the product of the 9E3 gene to show for the first time the distribution in vivo of the protein of one of these genes. The polyclonal antibody was produced against a synthetic peptide, [Cys76], 9E3, (77-103), located at the carboxy end of the molecule. The specificity of the antibody was determined by transfection of the 9E3 cDNA into Cos 7 cells, which do not express this gene. Moreover, despite the high homology between 9E3 and IL-8, the antibody did not crossreact with this molecule. The antibody was used to immuno-precipitate the protein from cultured normal and RSV-transformed chick embryo fibroblasts (CEFs) and to determine its distribution in tissues of newly hatched chicks. The staining was abundant in the cells and extracellular matrix (ECM) of connective tissue and other tissues of mesenchymal origin, such as bone and tendon. Most cells in the granulation tissue of wounds stained, some more intensely than others; the ECM also stained, especially in areas of scar tissue where collagen is abundant. In RSV-induced tumors, the protein was absent except in necrotic areas where a few cells--potentially macrophages--stained. In general, as expected, the protein was present in the cells and tissues that expressed the mRNA, but there were exceptions.(ABSTRACT TRUNCATED AT 250 WORDS)
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  Q.-J. Li, S. Vaingankar, F. M. Sladek, and M. Martins-Green Novel nuclear target for thrombin: activation of the Elk1 transcription factor leads to chemokine gene expression Blood, December 1, 2000; 96(12): 3696 - 3706. [Abstract] [Full Text] [PDF]
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 Q. Li, S. M. Vaingankar, H. M. Green, and M. Martins-Green Activation of the 9E3/cCAF Chemokine by Phorbol Esters Occurs via Multiple Signal Transduction Pathways That Converge to MEK1/ERK2 and Activate the Elk1 Transcription Factor J. Biol. Chem., May 28, 1999; 274(22): 15454 - 15465. [Abstract] [Full Text] [PDF]
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S. M. Vaingankar and M. Martins-Green Thrombin Aivation of the 9E3/CEF4 Chemokine Involves Tyrosine Kinases Including c-src and the Epidermal Growth Factor Receptor J. Biol. Chem., February 27, 1998; 273(9): 5226 - 5234. [Abstract] [Full Text] [PDF]
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 J. E. Feugate, Q. Li, L. Wong, and M. Martins-Green The cxc chemokine cCAF stimulates differentiation of fibroblasts into myofibroblasts and accelerates wound closure J. Cell Biol., January 7, 2002; 156(1): 161 - 172. [Abstract] [Full Text] [PDF]
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