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Journal of Cell Science, Vol 101, Issue 3 657-670, Copyright © 1992 by Company of Biologists
JOURNAL ARTICLES |
JF Collard, JL Senecal and Y Raymond
Institut du Cancer de Montreal, Hopital Notre-Dame, Quebec, Canada.
The nuclear lamina of mammalian somatic cells is characterized by the constitutive presence of lamin B polypeptides while the appearance of lamins A and C generally occur during establishment of a differentiated phenotype. We have used antibodies specific to the unique carboxy-terminal domain of lamin A, i.e. distinct from the shared domains of lamins A and C, to study the individual behaviour of lamin A during establishment of a macrophage phenotype in human HL-60 cells. Lamin A was present as a nuclear cap in the majority of undifferentiated cells and it was redistributed to a full peripheral nuclear location very early after induction of differentiation by phorbol esters, even in the presence of a protein synthesis inhibitor. Induction of the cells into a reversible precommitment state by bromodeoxyuridine was accompanied by a similar redistribution of lamin A that however reverted to a cap after removal of inducer. No changes were observed in the uniform peripheral nuclear location of lamin C under all of these conditions. These results strongly suggest that lamin A plays a role in the early events of cell differentiation. Taken together with previous results on the interaction of A-type lamins with chromatin, these findings offer experimental evidence consistent with the proposed role of A-type lamins, and particularly lamin A, in the process of chromatin reorganization that accompanies the expression of a differentiated phenotype.
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 B. Joseph, O. Lefebvre, C. Mereau-Richard, P.-M. Danze, M.-T. Belin-Plancot, and P. Formstecher Evidence for the Involvement of Both Retinoic Acid Receptor- and Retinoic X Receptor-Dependent Signaling Pathways in the Induction of Tissue Transglutaminase and Apoptosis in the Human Myeloma Cell Line RPMI 8226 Blood, April 1, 1998; 91(7): 2423 - 2432. [Abstract] [Full Text] [PDF]
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G. Pugh, P. Coates, E. Lane, Y Raymond, and R. Quinlan Distinct nuclear assembly pathways for lamins A and C lead to their increase during quiescence in Swiss 3T3 cells J. Cell Sci., January 10, 1997; 110(19): 2483 - 2493. [Abstract] [PDF]
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P Hozak, A. Sasseville, Y Raymond, and P. Cook Lamin proteins form an internal nucleoskeleton as well as a peripheral lamina in human cells J. Cell Sci., January 2, 1995; 108(2): 635 - 644. [Abstract] [PDF]
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A. Sasseville and Y Raymond Lamin A precursor is localized to intranuclear foci J. Cell Sci., January 1, 1995; 108(1): 273 - 285. [Abstract] [PDF]
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D. Lourim and J. J. Lin Expression of wild-type and nuclear localization-deficient human lamin A in chick myogenic cells J. Cell Sci., November 1, 1992; 103(3): 863 - 874. [Abstract] [PDF]
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