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Journal of Cell Science, Vol 101, Issue 3 647-655, Copyright © 1992 by Company of Biologists

JOURNAL ARTICLES

The interendothelial junction in myocardial capillaries: evidence for the existence of regularly spaced, cleft-spanning structures

C Schulze and JA Firth
Department of Anatomy and Cell Biology, St Mary's Hospital Medical School, London, UK.

Water and hydrophilic solutes cross the endothelium of continuous capillaries via the paracellular cleft and possibly other routes. This pathway shows a selectivity to molecule size and charge. However, it is not yet known which systems confer this selectivity. Isolated rat hearts were perfusion-fixed through the coronary circulation, stained with lanthanum or tannic acid, and further processed for transmission electron microscopy. Thin sections viewed at x 160,000 magnification revealed regularly spaced, cleft-spanning structures in the wider zone of a small percentage of clefts in addition to at least one zonula occludens. Goniometric tilting of the specimen in steps of 5 degrees perpendicular to the plane of the wide zone showed that such "linkers" can be revealed in at least 40% of all clefts. They become visible at some tilt angles, although the same area of the cleft is featureless at other angles. Single linker spacing measurements were obtained using a computerized image analysis system, and compiled in a frequency distribution chart. On the basis of these data, two models of a regular linker distribution within the cleft are illustrated. Our results provide evidence for the presence of regularly spaced, cleft-spanning structures within the interendothelial cleft which may have implications for endothelial cell-cell adhesion and permeability.


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© The Company of Biologists Ltd 1992