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Journal of Cell Science, Vol 101, 7-12, Copyright © 1992 by Company of Biologists
Submitted on July 24, 1991
Accepted on October 10, 1991
1 Molecular and Cellular Biology Program, University of Massachussetts, Amherst, Massachusetts 01003, USA
2 Department of Botany, University of Massachusetts, Amherst, Massachusetts 01003, USA
3 Molecular and Cellular Biology Program, University of Massachussetts, Amherst, Massachusetts 01003, USA; Department of Biochemistry, University of Massachussetts, Amherst, Massachusetts 01003, USA
4 Molecular and Cellular Biology Program, University of Massachussetts, Amherst, Massachusetts 01003, USA; Department of Botany, University of Massachusetts, Amherst, Massachusetts 01003, USA
Author for correspondence
Fluorescence ratiometric imaging of Lilium pollen tubes loaded with the Ca2+ indicator Fura-2 dextran has revealed a distinct elevation of free intracellular calcium ion concentration ([Ca2+]i) at the extreme tip of actively growing Lilium pollen tubes that declines to a uniform basal level of 170 nM throughout the length of the tube. The calcium gradient occurs within the first 10-20 µm proximal to the tip. Experimental inhibition of tip growth, usually achieved through the injection of the Ca2+ buffer 5,5'-dibromo BAPTA, results in the loss of the [Ca2+]i gradient. Occasionally these inhibited cells reinitiate growth, and when they do so ratio imaging reveals that the tip gradient of free [Ca2+]i re-emerges. The results presented here are very different from those previously published by revealing the presence of the [Ca2+]i gradient that is restricted to the 10-20 µm adjacent to the tube tip. Further, these experiments demonstrate a strict correlation between the presence of a [Ca2+]i gradient, and tip growth in Lilium pollen tubes.
Key words: free Ca2+ gradient, Fura-2 dextran, pollen tubes, polarity, tip growth
Submitted on July 24, 1991
Accepted on October 10, 1991
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