spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by J. HOEIJMAKERS, J. H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by J. HOEIJMAKERS, J. H.

Journal of Cell Science, Vol 100, 687-691, Copyright © 1991 by Company of Biologists

How Relevant is the Escherichia coli UvrABC Model for Excision Repair in Eukaryotes?

J. H. J. HOEIJMAKERS 1

1 Department of Cell Biology and Genetics, Medical Genetics Centre, Erasmus University, P.O. Box 1738, 3000 DR Rotterdam, The Netherlands

Knowledge about the DNA excision repair system is increasing rapidly. A detailed model for this process in Escherichia coli has emerged in which a lesion in the DNA is first recognized by the UvrA2B helicase complex. Subsequently, UvrC mediates incision on both sites of the DNA injury. Finally, the concerted action of helicase II (UvrD), polymerase and ligase takes care of removal of the damage-containing oligonucleotide, DNA resynthesis and sealing of the residual nick.

In the eukaryotes, yeast and mammals a total of 10 excision repair genes have been analysed thus far. However, little is still known about the molecular mechanism of this repair reaction. Amino acid sequence comparison suggests that at least three DNA helicases operate in eukaryotic nucleotide excision. In addition, a striking sequence conservation is noted between human and yeast repair proteins. But no eukaryotic homologs of the UvrABC proteins have been identified. In this Commentary the parallels and differences between the prokaryotic and eukaryotic excision repair pathways are weighed in an attempt to assess the relevance of the E. coli model for the eukaryotic system.

Key words: DNA excision repair, evolutionary conservation, DNA repair genes




This article has been cited by other articles:


Home page
 Cold Spring Harb Symp Quant BiolHome page
J.T. Reardon, L.H. Thompson, and A. Sancar
Excision Repair in Man and the Molecular Basis of Xeroderma Pigmentosum Syndrome
Cold Spring Harb Symp Quant Biol, January 1, 1993; 58(0): 605 - 617.
[Abstract] [PDF]


© The Company of Biologists Ltd 1991