spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Grunt, T. W.
Right arrow Articles by Dittrich, C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Grunt, T. W.
Right arrow Articles by Dittrich, C.

Journal of Cell Science, Vol 100, Issue 3 657-666, Copyright © 1991 by Company of Biologists

JOURNAL ARTICLES

The effects of dimethyl sulfoxide and retinoic acid on the cell growth and the phenotype of ovarian cancer cells

TW Grunt, C Somay, M Pavelka, A Ellinger, E Dittrich and C Dittrich
Department of Internal Medicine I, University of Vienna, Austria.

We have compared the in vitro effects of the differentiation inducers dimethyl sulfoxide (DMSO) and retinoic acid (RA) on a polyclonal human ovarian cancer cell line (HOC-7). Density gradient fractionation of untreated cells reveals that a proportion of rapidly growing, polygonal cells with medium density is capable of spontaneous reversion into a slowly growing low-density phenotype with flattened morphology similar to non-transformed human ovarian surface epithelial cells. Clonal expansion of these low-density cells proves that the observed characteristics are stable for prolonged culture periods. Exposure of HOC-7 cells to DMSO and RA or removal of the serum from the medium is effective in enhancing the proportion of these low-density cells. Application of DMSO causes the cells to become flattened and elongated, and to develop rod-like protrusions. In these cytoplasmic extensions thick filament bundles are dominant. Immunofluorescence studies demonstrate that both untreated low-density subclones and DMSO-treated polyclonal cells are much more reactive for cytokeratin than medium-density subclones or untreated parental cells. Furthermore, immunocytochemistry and fixed-cell ELISA reveal 2- to 5-fold greater amounts of desmoplakins I and II and of fibronectin in low-density subclones and in DMSO-treated cells as compared to medium-density subclones and control cultures. RA exerts weaker effects on the phenotype of the cells. Both inducers reduce DNA synthesis and inhibit the anchorage-dependent and the anchorage-independent cell growth in a dose- and time-dependent manner. The restoration of the original morphology and growth rate after removal of the differentiation-inducing agents proves that the observed changes are reversible; this indicates that the cells do not become terminally differentiated.(ABSTRACT TRUNCATED AT 250 WORDS)


This article has been cited by other articles:


Home page
 J. Nutr.Home page
K. J. Soprano and D. R. Soprano
Retinoic Acid Receptors and Cancer
J. Nutr., December 1, 2002; 132(12): 3809S - 3813.
[Abstract] [Full Text] [PDF]

Home page
 J. Nutr.Home page
P. Prakash, T. G. Manfredi, C. L. Jackson, and L. E. Gerber
{beta}-Carotene Alters the Morphology of NCI-H69 Small Cell Lung Cancer Cells
J. Nutr., January 1, 2002; 132(1): 121 - 124.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
T. W. Grunt, C. Somay, H. Oeller, E. Dittrich, and C. Dittrich
Comparative analysis of the effects of dimethyl sulfoxide and retinoic acid on the antigenic pattern of human ovarian adenocarcinoma cells
J. Cell Sci., October 1, 1992; 103(2): 501 - 509.
[Abstract] [PDF]


© The Company of Biologists Ltd 1991