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Journal of Cell Science, Vol 100, 501-507, Copyright © 1991 by Company of Biologists

Submitted on July 9, 1991
Accepted on August 9, 1991

Different Patterns of {alpha}-Tubulin Post-Translational Modification in Ovarian Nutritive Tubes of Two Hemipteran Insects

ALISTAIR HARRISON 1, HOWARD STEBBINGS 2, and JEREMY S. HYAMS 1

1 Department of Biology (Darwin Building), University College London, Gower Street, London WClE 6BT, UK
2 Departrnent of Biological Sciences, Washington Singer Laboratories, University of Exeter, Perry Road, Exeter EX4 4QG, UK

Author for correspondence

Usage of the tyrosinated, detyrosinated and acetylated forms of {alpha}-tubulin in ovarian nutritive tube microtubules of the hemipterans Oncopeltus fasciatus and Notonecta glauca glauca was investigated by immunofluorescence microscopy of frozen sections of ovarioles with isotype-specific antibodies. In Oncopeltus, nutritive tubes at all stages of development contained tyrosinated {alpha}-tubulin and showed only a weak reaction to antibodies to the detyrosinated and acetylated forms. In Notonecta, tyrosinated {alpha}-tubulin was confined to a zone around the periphery of functional nutritive tubes; the body of these tubes, and the older, redundant, nutritive tubes stained strongly for both the detyrosinated and acetylated isotypes. The difference in isotype usage between the two species was confirmed by immunoblotting of 2-D gels of ovariole extracts. The results are consistent with the different time-course of oogenesis, and hence the longevity of the nutritive tube microtubules, in the two insects. A model for the insertion of new microtubules into nutritive tubes as they grow is proposed.

Key words: {alpha}-tubulin, ovarian tubes, hemipterans

Submitted on July 9, 1991
Accepted on August 9, 1991






© The Company of Biologists Ltd 1991