|
|
|
|
|
|
|
|
|||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | |||||
Journal of Cell Science, Vol 100, Issue 3 451-458, Copyright © 1991 by Company of Biologists
JOURNAL ARTICLES |
T Gilbert and E Rodriguez-Boulan
Department of Cell Biology and Anatomy, Cornell University Medical College, New York, NY 10021.
Complete disruption of the microtubular network by colchicine or nocodazole in Caco-2 intestinal epithelial cells results in the appearance of basolateral microvilli and brush border-containing intracellular vacuoles (vacuolar apical compartment: VAC). These vacuoles are surrounded by a terminal web, express apical markers and exclude basolateral markers. The vacuoles do not originate from internalized apical or basolateral plasma membrane and their development is blocked by protein synthesis inhibitors, suggesting that they are newly synthesized. After removal of the microtubule inhibitors, VACs are usually degraded and/or released into the lateral intercellular space. Rarely was fusion with the apical membrane observed. These experiments support a role for microtubules in the biogenesis of the apical surface and indicate that, under some conditions, apical plasma membrane assembly may occur in the cytoplasm, as observed in some human pathological states.
This article has been cited by other articles:
|
|
 |
|
  T. Yamanaka, Y. Horikoshi, N. Izumi, A. Suzuki, K. Mizuno, and S. Ohno Lgl mediates apical domain disassembly by suppressing the PAR-3-aPKC-PAR-6 complex to orient apical membrane polarity J. Cell Sci., May 15, 2006; 119(10): 2107 - 2118. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Utech, A. I. Ivanov, S. N. Samarin, M. Bruewer, J. R. Turner, R. J. Mrsny, C. A. Parkos, and A. Nusrat Mechanism of IFN-{gamma}-induced Endocytosis of Tight Junction Proteins: Myosin II-dependent Vacuolarization of the Apical Plasma Membrane Mol. Biol. Cell, October 1, 2005; 16(10): 5040 - 5052. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. L. Tuma, L. K. Nyasae, and A. L. Hubbard Nonpolarized Cells Selectively Sort Apical Proteins from Cell Surface to a Novel Compartment, but Lack Apical Retention Mechanisms Mol. Biol. Cell, October 1, 2002; 13(10): 3400 - 3415. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. H. Low, M. Miura, P. A. Roche, A. C. Valdez, K. E. Mostov, and T. Weimbs Intracellular Redirection of Plasma Membrane Trafficking after Loss of Epithelial Cell Polarity Mol. Biol. Cell, September 1, 2000; 11(9): 3045 - 3060. [Abstract] [Full Text]
|
|
|
|
 |
|
 C. YEAMAN, K. K. GRINDSTAFF, and W. J. NELSON New Perspectives on Mechanisms Involved in Generating Epithelial Cell Polarity Physiol Rev, January 1, 1999; 79(1): 73 - 98. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M Brignoni, O. Pignataro, M. Rodriguez, A Alvarez, D. Vega-Salas, E Rodriguez-Boulan, and P. Salas Cyclic AMP modulates the rate of 'constitutive' exocytosis of apical membrane proteins in Madin-Darby canine kidney cells J. Cell Sci., January 5, 1995; 108(5): 1931 - 1943. [Abstract] [PDF]
|
|
|
|
|
|
M. Peterson, W. Bement, and M. Mooseker An in vitro model for the analysis of intestinal brush border assembly. II. Changes in expression and localization of brush border proteins during cell contact-induced brush border assembly in Caco-2BBe cells J. Cell Sci., January 6, 1993; 105(2): 461 - 472. [Abstract] [PDF]
|
|
|
|
|
|
G. K. Ojakian and R. Schwimmer Antimicrotubule drugs inhibit the polarized insertion of an intracellular glycoprotein pool into the apical membrane of Madin-Darby canine kidney (MDCK) cells J. Cell Sci., November 1, 1992; 103(3): 677 - 687. [Abstract] [PDF]
|
|
