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Figure 1


Fig. 1. Morphology of the cysts and polarization of different markers in the control and crumbs-3 knockdown (Crb-3 KD) cells. (A-H) Control and Crb-3 KD cysts (A-D and E-H, respectively) were immunostained with antibodies against the apical markers podocalyxin (Pcx) or carcinoembryonic antigen (CEA), the basolateral membrane marker E-cadherin (E-cad), or ZO-1 for cell junctions as indicated. AlexaFluor®488-conjugated secondary antibodies were applied to visualize the different markers in green. All cysts were also stained for actin (TRITC-phalloidin; red) indicating outlines of lumina and individual cells. DAPI staining (blue) shown nuclei. Arrowheads in G indicate aberrant localization of E-cadherin at the luminal surface in a Crb-3 KD cyst. Scale bars, 30 µm.