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Figure 1


Fig. 1. Characterization of primary MTEC. (A) Compared to isolated lung fibroblasts, MTEC expressed cytokeratins and E-cadherin, but were negative for fibronectin and {alpha}-SMA. (B) MTEC expressed the localized tight junctional protein ZO-1, indicative of barrier function (ZO-1 is depicted by the red stain, whereas the green signal represents the DNA counterstain, Sytox Green). (C) Scanning electron micrograph of MTEC depicting well-defined cell-cell contacts, 2500x magnification. (D) Transmission electron micrograph depicting tight junction formation, desmosomes, nuclei, vacuole; 15000x magnification. (E) Primary MTEC established stable TER in culture. Shown are MTEC cultured under submerged conditions, or cultured on ALI in the presence of absence of retinoic acid (30 nM); TER was measured every other day for 18 days. D, desmosome; N, nucleus; T, tight junction; V, vacuole.