Fig. 4. Grh expression results in altered cellular morphology and disrupts the distribution of integrins and cadherin. Dorsal views of control (A,B) and AS^c381>grh (C-F) embryos stained with antibodies against βPS integrin (green, A-D; white, A'-D') and E-cadherin (magenta, A-E; white, A''-D'',E',F). (A-D) AS^c381>grh cells have poor localisation and patchy accumulation of βPS integrin (e.g. arrows, C,C'), reduced βPS integrin levels at epidermal interface (arrowheads, C,C',D,D'), reduced co-localisation between βPS integrin and E-cadherin (compare B with D), and some mislocalisation of E-cadherin (e.g. arrows C''). (E,F) Defects in the epidermis include bunching (orange arrows) and splaying (green arrows) of epidermal cells, and a detachment between the epidermis and the amnioserosa. (E) Nuclei are visualised with DAPI.