Fig. 7. Dendritic cells were stained and observed as described in Fig. 5 to distinguish surface (red) and internalized (yellow) DC-SIGN. (A) DIC image of the dendritic cell under observation. (B) A single frame (from supplementary material Movie 5) showing the leading edge of the dendritic cell (yellow line), internalized DC-SIGN (blue boxes), and mobile surface DC-SIGN clusters (white and cyan arrows). (C) Trajectories followed by the mobile surface DC-SIGN clusters emphasized in (B) with trajectory start points indicated by green arrows. (D) Montage of selected frames showing the rapid linear mobility of these spots and their continued lack of colocalization with endocytosis marker (FITC-BSA). Scale bars, 1 µm.