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Figure 2


Fig. 2. DC-SIGN cluster photobleaching and recovery. (A) Montage showing the bleaching of a representative DC-SIGN cluster on an MX-DCSIGN cell stained with anti-DC-SIGN F(ab). The bleached region is indicated with a yellow box and bleaching occurred between the third and fourth frames. (B) The complete intensity profile in time of the bleached cluster. (C) A montage showing the bleaching of several DC-SIGN clusters on a dendritic cell. (D) Bleaching and recovery phase for a representative cluster on this dendritic cell. (E) Distribution of mobile fractions (Mf) as measured from the FRAP curves of all DC-SIGN clusters analyzed in both MX-DCSIGN cells and dendritic cells (n=7 in each cell type, error bars denote ± s.d.). Red and blue bars show the mean Mf for all trials per cell type. Scale bars, 1 µm.