JCS -- de Bakker et al. 121 (5): 627 Figure IG6

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Fig. 6. High-resolution dual-color excitation-detection NSOM of two different cells reflecting nanometer-scale spatial colocalization of IL2R ${alpha}$ (labeled with Alexa-Fluor-488-conjugated 7G7-B6 mAb) and IL15R ${alpha}$ (labeled with Cy5-conjugated 7A4 24 mAb) on the cell membrane. Here the pseudo-color scale refers to spectral contrast, with Alexa-Fluor-488 emission shown in green, whereas Cy5 emission is shown in red. The yellow color indicates colocalization of Alexa-Fluor-488 and the Cy5 signal. The degree of colocalization has been calculated using the Pearson's correlation coefficient over ten different cells imaged with NSOM (see Materials and Methods for details). Bars, 1 µm.