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Figure 1


Fig. 1. Bid- and tBid-induced cytochrome c release from B50 cell mitochondria. Mitochondria were incubated for 30 minutes with either Bid or tBid, as indicated, and then sedimented. The mitochondrial pellets were analysed for cytochrome c in western blots using fluorescent secondary antibodies as shown. (Graph) Bands were quantified by fluorescence imaging and fractional cytochrome c release calculated (where 0-1 indicates 0-100% release). Cytc, cytochrome c.