Fig. 7. Immunofluorescent labeling of FACS-sorted UI cells and murine HFs. (A-D) 6 integrin (A,C) and K14 (B,D) immunofluorescence in cytospun UI and IFE + IFD FACS-sorted cells. Arrowheads indicate polarized surface 6 or cytoplasmic K14 expression. (E) 6 integrin and CD44 immunofluorescence in a HF. (F-H) Tbc1d10c immunofluorescence in cryosections of adult mouse skin. Three separate HFs are shown. Arrowheads indicate positive Tbc1d10c labeling in the UI region and a hashed line demarcates the basal layer of the IFE (I,K). Staining in the cornified IFE was also observed in control sections stained with secondary antibodies alone (data not shown). BG, bulge; hs, hair shaft; sg, sebaceous gland. Scale bars: 100 µm.