Fig. 6. Verification of enriched transcripts in UI cells by RT-PCR analysis. (A) Total RNA from FACS sorted UI, bulge and IFE + IFD epidermal cells was used to generate first-strand cDNA for semi-quantitative RT-PCR analysis using primers for genes enriched in UI cells (Table 2). RT-PCR for GAPDH was conducted as an internal control. Each RT-PCR reaction was generated from undiluted (1), 1:5 dilution (1/5) or a 1:25 dilution (1/25) of each cDNA sample. No amplification products were observed in the water control (C) samples. (B) Quantification of RT-PCR transcript levels generated from undiluted cDNA samples using NIH ImageJ software. Data are expressed as arbitrary densitometric units relative to GAPDH levels.