Fig. 5. Cell cycle status and label retention in UI keratinocytes. (A-F) FACS dot blot showing DNA content (y-axis) vs pulse width (x-axis) from FACS-sorted keratinocytes isolated from telogen (A,C,E) and anagen (B,D,F) skin. (G) Quantification of G0-G1, S or G2-M phase cells. (H) Cells retaining label are represented as the average percentage of BrdU-positive cells for each FACS-sorted population within the total number of BrdU-positive cells in the entire epidermis per experiment. 2000 cells per population were counted in each experiment and data represent the average of two experiments. *P < 0.05, statistically significant from UI and IFE + IFD cells (Student's t-test). Error bars denote s.d.