Fig. 3. Colony-forming efficiency of UI epidermal keratinocytes. (A-C) All six FACS-sorted cell populations from Fig. 2 were plated at equal density and cultured for 2 weeks. Cultures were stained with Rhodamine and the average (n=3 plates per group) total colony number (B) and the percent of colonies >4 mm in size (C) were quantified using NIH ImageJ software and statistically compared using a Student's t-test (*P < 0.05, statistically different compared with UI cells; error bars represent s.d.). (D-F) Higher-magnification phase-contrast images of three FACS-sorted populations shown in A.