Fig. 2. High-magnification view of the hair follicle UI region and FACS analysis of UI epidermal keratinocytes from murine skin. Epidermal whole mounts were probed with antibodies to 6 integrin (A), Sca-1 (B) and CD34 (C). Merged panel (D) shows high magnification image of three HF compartments including 6^L+HCD34^–Sca-1⁺ IFE + IFD (P1), 6^LCD34^–Sca-1^– UI (P2) and 6^L+HCD34⁺Sca-1^– (P3) regions isolated by FACS below (E-M). (E-M) Keratinocytes were isolated from adult dorsal skin as previously described (Morris, 1994), labeled with 6 integrin, CD34 and Sca-1 antibodies and subjected to flow cytometry. Viable 6-positive cells were identified and further gated based on their surface expression of CD34 and Sca-1. Scale bar: 20 µm.