Fig. 4. Nuclear reorganisation and chromatin decondensation in the limb bud of E9.5 Hoxb1-LacZ embryos. (A) Histograms showing the percentage of Lnp, Hoxb1-LacZ or Hoxd hybridisation signals inside, at the edge or outside of the MMU2 CT in the forelimb bud of E9.5 Hoxb1-LacZ embryos (black bars) compared with the endogenous Hoxd (white bars). The cut-offs used for the edge category are 0.4 µm and –0.4 µm from CT edge. (B) Distributions of squared interphase distances (d² in µm²) standardised to genomic separation (in Mb) measured between Lnp and Hoxd probes (white bars) at endogenous Hoxd in forelimb bud nuclei from E9.5 wild-type embryos or between the Lnp and Hoxb1-LacZ probes (grey bars) and between the Hoxb1-LacZ and Hoxd probes (black bars) in forelimb nuclei from E9.5 Hoxb1-LacZ embryos.