JCS -- Besteiro et al. 121 (5): 561 Figure IG4

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Fig. 4. Acidocalcisomal membrane V-H⁺-PPase is downregulated in ${Delta}$ ap3 ${delta}$ promastigotes. (A) Western blot analysis of whole-cell lysates from L. major promastigotes with the anti-V-H⁺-PPase antibody (VP1). Elongation factor 1 ${alpha}$ (EF1- ${alpha}$ ) was used as a loading control. Molecular sizes are indicated on the left. (B) Immunofluorescence analysis of L. major promastigotes using the anti-V-H⁺-PPase antibody. (C) Localisation in WT and ${Delta}$ ap3 ${delta}$ L. major promastigotes, of the V-H⁺-PPase with GFP fused at its C-terminal end. All scale bars, 10 µm; n, nucleus; k, kinetoplast. Corresponding DIC images are shown as well.