JCS -- Heit et al. 121 (2): 205 Figure IG3

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Fig. 3. The role of PI3K ${delta}$ and PI3K ${gamma}$ in mediating initial responses to fMLP. Neutrophils were either uninhibited human cells (uninhibited), uninhibited murine cells (C57Bl/6), treated with a PI3K ${delta}$ -specific inhibitor (IC87114) or isolated from PI3K ${gamma}$ ^–/– mice. fMLP was added at time=0 and the cells monitored for 20 minutes. (a) Percentage of human cells that polarized in response to fMLP. (b) Percentage of human neutrophils orientated towards fMLP. (c) Speed of human neutrophil migration in response to fMLP. (d) Percentage of murine cells that polarized in response to fMLP. (e) Percentage of murine neutrophils orientated towards the source of fMLP. (f) Speed of murine neutrophil migration in response to fMLP. (g) Percentage of murine cells that polarized in response to fMLP. (h) Percentage of murine neutrophils orientated towards the source of fMLP. (i) Speed of murine neutrophil migration in response to fMLP. (j) Adhesion of murine neutrophils to ICAM1 pre- and post-fMLP stimulation. n=4 for all data.