Fig. 5. Axonal respecification is preceded by the reversion of membrane flow and does not involve rotation of the centrosome. (A) Live cell analysis of post-Golgi membrane traffic visualized by labelling with BODIPY-ceramide after recovery from axotomy (2-3 hours after cutting: arrow) shows that more traffic is directed towards the opposite side (open arrowheads) from the sectioned axon (original axon at 0': arrowheads, n=15). (B) Neurons were transfected with centrin-1–GFP to mark the centrosome position (open arrowhead), and the centrosome was observed before axotomy and after axon regrowth. Arrowheads, axons; arrow, axotomy; open arrowheads, centrin-1–GFP. Bars, 10 µm. (C) Quantification of the results in B: frequency distribution showing centrosome position after axotomy with respect to its original position when the axotomy was performed (bin size 10°; n=26 neurons).