Fig. 5. The pleiotropic effects of ICL1ep depletion. Cells were observed by labelling with either the anti-centrin antibody 1A9 to visualise the ICL (A) or with the anti-tubulin ID5 to visualise microtubular structures and in particular basal bodies (B-C). (A) Disassembly of the ICL after 24 hours of ICL1ep depletion shows a distinctive pattern compared with those in Fig. 4. No terminal phenotype with complete absence of ICL was observed, because cells die after a few divisions under RNAi conditions. (C) Disorganisation of the cortex, observed after 48 hours. Comparison with a control cell (B) shows that ICL1ep depletion leads to fewer and misaligned basal bodies, abnormal and reduced oral apparatus (oa) and post-oral microtubules (pof) and an abnormal number of contractile vacuole pores (cvp). Scale bar: 15 µm.