Fig. 3. Localisation of GFP-tagged centrins. For a representative of each centrin subfamily, localisation was monitored in GFP-expressing cells, fixed and double-labelled with an anti-GFP polyclonal antibody (green) and either the monoclonal anti-centrin1A9, specific for the ICL (red) or the monoclonal ID5, which labels essentially the basal bodies (red). To the right of each represented cell, the insets show, from top to bottom, GFP, anti-centrin or anti-tubulin labelling and the merged image of a magnified area. (A) In cells expressing ICL1ap-GFP, the two labels precisely colocalise. (B) In cells expressing ICL10ap-GFP, the GFP labelling clearly shows a beaded pattern, whereas the anti-centrin labelling (specific for the ICL1a subfamily) is continuous along the mesh. (C) In cells expressing ICL1ep-GFP, the GFP signal localises not only at the ICL, with a beaded pattern, but also at the contractile vacuole pores (arrowheads), and anteriorly, in close association with basal bodies (arrows). Scale bar: 15 µm.