(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.


Figure 1


Fig. 1. Expression of transgenic {alpha}-dystrobrevin in skeletal muscle. (A) The murine creatine kinase (MCK) promoter was used to drive expression of {alpha}-dystrobrevin-2a linked to either HA at the C-terminus or to HA followed by the Ras palmitoylation site (RPS), to force membrane association. (B) Immunoblotting of muscle homogenates using an anti-dystrobrevin antibody show the relative transgene expression of the three founder lines of TgDB (63, 68, 80), five lines of TgDB-RPS (10, 17, 27, 29, 71) and wild type (wt). The bottom part of the same immunoblot was labeled with an antibody against glyceraldehyde-3-phosphate dehydrogenase (GAPDH) to demonstrate that similar amounts of protein were loaded on the gel.