Fig. 4. Mbx1p binds to Plo1p throughout the cell cycle. cdc25-22 mbx1-13myc cells were synchronised by transient temperature arrest and samples taken every 20 minutes after returning the cells to the permissive temperature. Septation indices were counted microscopically and are plotted to indicate the synchrony of the culture. Soluble extracts from each time point were prepared, Plo1p was immunoprecipitated with antibody against the native protein, and the immunoprecipitates analysed by western blotting with antibodies against Plo1p and Myc (the latter to detect Mbx1p-13myc). asy, control sample from asynchronous cells prior to synchronisation. Quantification of co-immunoprecipitated Mbx1p against immunoprecipitated Plo1p is shown.