Fig. 7. Chromosome-binding assay showing that lamin Dm₀ binding to histone H2A requires both the N- and C-tail domains of histone H2A. The lamin Dm₀ tail domain (residues 425-622) was used to bind mitotic CHO chromosomes in the presence of 10% BSA with or without 100-fold molar excess of competitor histone H2A-derived proteins. Chromosomes were co-stained with DAPI (left) and with 611A3A6 monoclonal anti lamin Dm₀ antibody as primary antibody and Cy3-conjugated anti-mouse antibodies as secondary antibodies (right). The control did not include the lamin Dm₀ protein. Quantification of the fluorescent signal was performed using the Science Laboratory 99 Image Gauge software and is given as the average fluorescence signal of binding to mitotic chromosomes in arbitrary units/sq. pixel. Data was taken from at least three independent experiments. SD, standard deviation.