Fig. 2. The binding of lamin Dm₀ to chromosomes requires both the KRKR (NLS) and the RAT sequences. (A,B) The lamin Dm₀ tail domains (residues 425-622), wild-type and containing the indicated mutations, were used to bind mitotic CHO chromosomes in the presence of 10% BSA. The control did not include the lamin Dm₀ protein. Chromosomes were co-stained with DAPI (left) and with 611A3A6 monoclonal anti-lamin Dm₀ antibody as primary antibody and Cy3-conjugated anti-mouse antibodies as secondary antibodies (right). Quantification of these experiments is shown in Table 1.