Fig. 6. Temporal mitotic distribution of Sp3 and lamins. MCF-7 cells were grown on coverslips in estrogen-complete medium, fixed, and double labeled with rabbit anti-Sp3 antibodies and mouse monoclonal anti-lamin A/C antibodies. DNA was stained by DAPI. Spatial distribution was visualized by fluorescence microscopy and image deconvolution as described in Materials and Methods. Single optical sections are shown. Bar, 5 µm.