Fig. 1. Anti-peptide ChlR1 2075 antibody specifically immunoprecipitates ChlR1 protein. FLAG-ChlR1 was in vitro translated using a TNT kit (Promega) and the protein was used for immunoprecipitation with ChlR1 2075 or previously characterized Hel1 antibody (Amann et al., 1997). (B) Cells were transfected with FLAG-ChlR1 and metabolically radiolabeled. Whole cell lysate was used for immunoprecipitation with either ChlR1 2075 antisera or Hel1 (Amann et al., 1997). A band corresponding to the predicted molecular mass of ChlR1 (~102 kDa) was specifically immunoprecipitated with both antibodies. (C) HeLa cell lysates were used to immunoprecipitate endogenous ChlR1 protein with ChlR1 2075 antibody followed by detection by western analysis with the Hel1 antibody.