Fig. 6. Constitutively active RhoA rescues p38 activity in dominant-negative N-cadherin transfected myoblasts. (A) Western blot detection of phospho-p38 (pp38), phospho-ERK1/2 (pERK1/2), total ERK1/2, HA and Myc tags, total p38 and ß-actin in lysates from C2 cells that were transfected with pcDNA, cN390, or co-transfected with cN390 and L63 RhoA. Phospho- and total p38 were semi-quantified by scanning densitometry, and p38 phosphorylation normalised for total p38 (lower panel); data are expressed as fold increases of p38 phosphorylation above pcDNA control levels; ^*P<0.05; ^***P<0.001 compared with levels in the control. (B) p38 MAP kinase activity (MBP phosphorylation) in lysates from C2 cells treated as in A; total p38 levels were determined by western blotting.