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Figure 6


Fig. 6. Heterologous expression of claudins in transfected COS-7 cells. (a-c) Freeze fracture of unfixed transfected COS-7 cells shows fibrils (black arrowheads) for GFP-claudin-9 (a), -6 (b) and -14 (c) on the P- and E-fracture faces. (d-f) Confocal colocalization (white arrowheads) of endogenous ß-catenin (red) with GFP-claudin-9 (d) and -6 (e) but not -14 (f) (green) at transfected cell-cell contacts. Arrows in (d), GFP-claudin-9 in filopodia of a transfected cell. (g-i) Confocal colocalization (white arrowheads) of endogenous p120ctn (red) and GFP-claudin-9 (d) and -6 (e) but not -14 (f) (green). Bar in i, 0.15 µm (a-c); 3.4 µm (d-i).