Fig. 6. PKC phosphorylates N-terminal Ser residues of ß-catenin in vitro. GST-ß-catenin (100 ng) was incubated with the indicated amount of purified PKC (A) or BIM (0.5 µM; B), and then the samples were analyzed by western blotting with anti-phospho-p45-ß-catenin and anti-phospho-p33/37-ß-catenin antibodies.