Fig. 3. The N-terminus of ß-catenin is required for A23187-mediated ß-catenin degradation. HEK293 cells were transfected with wild-type ß-catenin (A), ß-catenin (B) or ß-catenin (S37A) (C) plasmids, incubated with A23187 [1.25 (+) and 2.5 (++) µM] for 15 hours, and then cytosolic proteins were immunoblotted with anti-ß-catenin antibody. The blots were reprobed with anti-GFP antibody as a transfection control.