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Figure 2


Fig. 2. TAp63 isoforms induce IKK{alpha} protein expression. (A) Western blot in Saos-2 Tet-on inducible cell lines (Dox 2 µg/ml) overexpressing TAp63 or {Delta}Np63 isoforms. All TAp63 isoforms show an induction of IKK{alpha} at the protein level (from 1.3- to 3.7-fold). The actin western blot is shown in the lower panel as a loading control. IKK{alpha} expression was normalised to actin by densitometry and results are reported as fold change over zero time (see graphics below actin bands). (B) {Delta}Np63 isoforms expression do not change ({Delta}Np63{alpha}) or produce a minor downregulation ({Delta}Np63ß and {gamma}) of IKK{alpha} protein after 12 hours of Dox induction. IKK{alpha} expression was normalised to actin by densitometry and results are reported as fold change over zero time. (C) Western blot in Saos-2 Tet-on inducible cell lines (Dox 2 µg/ml) overexpressing p53. p53 does not induce IKK{alpha} at either protein or RNA level (see also Fig. 1) after 6 hours and 12 hours of induction. IKK{alpha} expression was normalised to actin by densitometry and results are reported as fold change over the level at zero time. All results obtained from the densitometry are shown as mean ± s.d. from three independent experiments.