Fig. 3. Distribution of nuclei in zygotes 3.5 hours after initiation of mating. Cultures of wild-type (HZH638 and HZH639) and mdy2 mutant (HZH686 and HZH683) strains were grown to log phase. MATa and MAT cells were mixed and incubated on YPD plates for 3.5 hours to allow zygotes to form. Cells were fixed, stained with DAPI (A), and the distance between the nuclei was measured using the centre of each nucleus as reference (n=85 unbudded zygotes for each strain). Bar, 5 µm.