Fig. 8. Erasin is found in a complex with other ERAD components. (A) Lysates from HeLa cells transfected with a gp78-expression construct or which were left untransfected were used to conduct immunoprecipitations with anti-erasin 130 antibody or its preimmune serum. The immunoprecipitates were immunoblotted with anti-gp78 (upper panel) and anti-erasin antibodies (second panel). The levels of expression of gp78, erasin and actin in equal amounts of protein lysate from these cultures are shown in the bottom three panels. (B) Immunoprecipitation of proteins from untransfected HeLa cells using anti-Derlin-1 antibody or a control IgG antibody and immunoblotted for erasin (upper panel) or Derlin-1. (C) Immunoblot analyses of equal amounts of proteins from normal HEK293 cells (control) and in cells in which erasin levels were reduced using siRNA, probed for the different proteins as listed.