Fig. 6. Schwann cells in the distal stump of nerves of GFAP-null mice after injury show reduced proliferation but normal apoptosis. (A-D) Nuclei staining with DAPI (blue) and BrdU (green) on longitudinal sections of the distal stump of the sciatic nerve at 3 and 6 days (t3 and t6, respectively) after injury. The number of BrdU-positive nuclei is reduced at both t3 and t6 in nerves of mutant mice. (E) Quantitative analysis shows that the percentage of BrdU-positive nuclei is significantly decreased at t3 (^*P=0.04) and consistently but not significantly reduced at t6 (P=0.06). (F-I) Staining of nuclei with DAPI (blue) and of phosphorylated histone H3 (green) on longitudinal sections of the distal stump of the sciatic nerve at days 3 and 6 after injury. The number of nuclei positive for phosphorylated histone H3 is reduced at both t3 and t6 in nerves GFAP-null mice. (J) Quantitative analysis shows that the percentage of phosphorylated histone H3 nuclei is significantly reduced at both t3 (^**P<0.001) and t6 (^*P=0.04). (K-N). S100 (green) and TUNEL (red) staining on longitudinal sections of the distal stump of the sciatic nerve at t3 and t6 after crushing. TUNEL staining shows a similar number of positive nuclei in mutant and control nerves at both time points. (O) Quantitative analysis shows no significant difference in the percentage of positive nuclei in mutants and controls. Error bars represent the ±s.e.m. Bar in N^I, 40 µm for A-D; 80 µm for F-I and K-N.