Fig. 8. PIKfyve suppression alters the steady-state distribution of not only CI-MPR but also sortilin and furin. (A-C) HeLaM cells stably expressing either (A) CD8-CI-MPR, (B) CD8-sortilin or (C) CD8-furin, were treated with control siRNA or a combination of PIKfyve duplexes II and V as described in Materials and Methods. Cells were then fixed and co-stained for CD8 and EEA1 prior to confocal imaging. Images are projections of at least eight confocal sections and are representative of over 40 cells imaged for each condition. Bars, 40 µm.