Fig. 10. AF6i1 and AF6i3 protein in Ca²⁺ switch assay: crucial role of the F-actin binding site. (A) Anti-ß-catenin staining of control cells, AF6i3-knockdown cells and knockdown cells reconstituted with AF6i1 or AF6i3 construct, 2 hours after switching to 2 mM Ca²⁺. Bar, 30 µm. (B) Graphic representation of the percentage of ß-catenin-positive cell-cell contacts shown in A. Values for ±s.d. were derived from three independent experiments. Total numbers of counted cell-cell contacts are indicated (n). Similar results were obtained for E-cadherin, nectin-1 and -catenin. (C) Length distribution of ß-catenin-positive cell-cell contacts shown in A, determined as described for Fig. 9D.