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Figure 3


Fig. 3. ADAM22 interacts with all 14-3-3 protein family members expressed in the brain. HEK 293T cells co-expressing FLAG-tagged full length ADAM22v4 and Myc-tagged full length 14-3-3 proteins ß, {gamma}, {epsilon}, {zeta}, {eta} or HA-tagged 14-3-3{tau} were lysed and proteins were immunoprecipitated (IP) with anti-Myc or anti-HA antibodies and probed with anti-FLAG antibody. Immunoblots were then re-probed with anti-Myc or anti-HA antibodies.