Fig. 4. In control G2 cells, the normal PML-NBs colocalise with juxtacentromeric satellite DNA, adjacent to the centromeric DNA. Immuno-FISH experiments demonstrate that PML protein signals (red) (A) are adjacent to the pan-centromeric DNA signals (cen, green) and (B) colocalise with the 1qh and 9qh satellite (1qh sat and 9qh sat) DNA signals (green). (C) Histogram showing the percentage of 1qh (M=24.64), 9qh (M=25.47), 16qh (M=6.5) and 15ph (M=4.44) satellite DNAs that colocalise with PML-NBs in three control subjects (C1, C2, C3) at G2 phase. The colocalisations have been scored from the analysis of 100 G2 cells and compared with those occurring between PML-NBs and chromosome 1 centromeric DNA, which is excluded from PML-NBs; P values were calculated by using Fisher's exact test, one sided (http://www.matforsk.no/ola/fisher.htm). M, mean.