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Fig. 6. Requirements for PLK1 downregulation in NARF2 cells. The expression levels of the indicated proteins were determined using western blot with extracts of NARF2 cells infected with viruses encoding the indicated genes. LXSN, pBabe-Puro and LV serve as controls. The NARF2 populations were exposed to IPTG for 48 hours and analyzed using antibodies to PLK1, p53, p21/WAF1, p14ARF and actin.