Fig. 8. Accumulation of abnormal cortical actin structures in response to lowered Alix levels. HeLa cells treated with control RNA (A-C) or with siRNA (D-F) against Alix (see Fig. 1B) were stained with rhodamine-phalloidin (B-E), anti-cortactin (C-F) and anti-EEA1 (A-D). Yellow shows colocalisation between actin and cortactin in abnormal actin structures. Scale bars: 5 µm.