Fig. 2. Alix depletion alters the distribution of endosomes. HeLa cells were treated with control RNA (A-D,J) or with siRNA against Alix (E-I) as in Fig. 1B. The cells were permeabilised before fixation and were stained with anti-EEA1 (A,E,I,J), anti-Hrs (B,F), anti-LBPA (C,G), or anti-LAMP1 (D,H). Scale bars: 5 µm. Asterisks in I,J represent nuclei.