Fig. 6. Inhibition of Rho-kinase or nonmuscle myosin II slightly increases protrusive activity and slightly decreases the regularity of wound closure. Progress of wound closure in MDCK cell monolayers in the presence or absence of H1152 (A) or (±)-blebbistatin (B). The initial diameter of the circular wounds was 500 µm. Lamellipodial density at 6 hours after wounding (number of lamellipodia at the wound margin and dividing by margin perimeter length) in the presence or absence of H1152 (C) or (±)-blebbistatin (D). Regularity of wound closure (percent increase from 0 to 6 hours after wounding in the quotient of margin perimeter length divided by area) in the presence or absence of H1152 (E) or (±)-blebbistatin (F). Data are mean and s.e.m. for n=12 individual wounds in separate wells on multiwell tissue culture plates from three independent experiments for each treatment at each indicated concentration.